To further assess the NF-κB(p65) and GLI1 transcriptional activity in AI versus AD prostate cell lines, the NF-κB(p65) DNA binding assay (Figure 3D) and luciferase reporter assay specific for NF-κB(p65) (Figure 3E) and GLI1 (Figure 3F) were performed. Here, NFKB1 is linked to Alzheimer disease.