In a single-cell transcriptomics analysis of the hypothalamus, it has been shown that, in long-term diet-induced obesity, the expression of CREB is reduced in AgRP/NPY neurons [16]; however, no previous study has evaluated the impact of the short-term consumption of an HFD on Pomc CREB. This evidence concerns the gene AGRP and obesity due to melanocortin 4 receptor deficiency.