Therefore, miR-186 targets Twist1 to inhibit the expression of N-cadherin, vimentin, and matrix metallopeptidase 9 (MMP9), as well as increase the expression abundance of E-cadherin to inhibit the proliferation, migration, invasion, and EMT of cholangiocarcinoma cells [89]. The gene discussed is MMP9; the disease is cholangiocarcinoma.