EIF4EBP1 and neuroblastoma: Since VLX600 was reported to inhibit mTOR signaling resulting from the mitochondrial inhibition-induced energy crisis, we performed an immunoblotting assay to determine the level of p-4EBP1/t-4EBP1 after incubation of VLX600, and we noticed a reduction in the ratio of p-4EBP1/4EBP1 in MYCN-amplified neuroblastoma cells (Figure 4A,B), revealing a downregulation of the mTOR pathway.