Proteomic analysis following Twist-mediated induction of EMT in MEC results in activation of FAK/ILK, mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK), PI3K/Akt, and Wnt signaling, while depletion of ILK curtails Twist-mediated EMT and invasion [61], and depletion of Twist in breast cancer cells decreases activation of FAK/ILK and downstream signaling, leading to reduced EMT and invasion [61]. The gene discussed is TWIST1; the disease is breast cancer.