Of note, Li et al., showed that TIM-3 is also highly expressed on γδ T cells, which represent a low population of CD3+CD4−CD8− T cell populations in the TME, in CRC patients, and attributed reduction in perforin/granzyme B production in γδ T cell subtypes to TIM-3 expression [44]. Here, PRF1 is linked to colorectal carcinoma.