The effects of GSN and PRDX4 on biological processes relevant to the EGFR/RhoA/PKCα/ERK signaling pathways in CRC and the protein levels p-EGFR, p-RhoA, p-PKCα, and p-ERK1/2 in DLD-1 cells were widely used as a model for studies of cell signaling and invasion; therefore their expressions were determined in this study by western blot analysis. The gene discussed is RHOA; the disease is colorectal carcinoma.