Cryopreserved UCB CAR-T cells were able to recognize and target tumor cells, and they had the capacity to secrete IL-2 (26.0 ± 4.9%) and IFN-γ (19.9 ± 6.7%) in a similar manner to fresh UCB CAR-T (p = 0.63 for IL-2 and IFN-γ expression between fresh and tUCB CAR-T) (Figure 5G). Here, IFNG is linked to neoplasm.