Similar to parental HEK293 cells, 8C7-ADCs were ineffective against LIM1215 colon and U251 glioblastoma (GBM) tumour cells expressing ADAM10 (Figure S3), consistent with the low binding of 8C7 to live cells in vitro [19] compared to that in tumours, likely reflecting a predominantly inactive conformation on cells cultured in vitro [20]. Here, ADAM10 is linked to glioblastoma.