Among them, significantly altered genes were: GBM1: up-regulated genes: RYR1, HS6ST2, and C14orf132, down-regulated genes: TACSTD2, VAMP8, and BPIFA1; JHH520: up-regulated genes: PAWR, TIMP3, and HOXB7, down-regulated genes: ABCG2, TSACC, and PYY; BTSC233: upregulated genes: NCAN, SHC2, and NF1, downregulated genes: SDK1, TFPI2, and NBDY. Furthermore, KEGG pathway analysis showed that these differentially expressed genes (DEGs) were highly associated with pathways ranging from metabolism to cancer (Figure 4C). The gene discussed is BPIFA1; the disease is cancer.