TWIST1 and neoplasm: Then, to investigate the relationship between the marker gene expression levels in CTCs and tumor stage, we analyzed the mRNA expression levels of BIRC5, CDH11, EGFR, EPCAM, KRT18, SPP1, TRPC1, TRPC6, TRPV6, TRPM4, TWIST1, TNC, VEGFA, VIM, and ZEB1 in the CTCs from the NMIBC and MIBC patients (Figure S2).