TWIST1 and breast cancer: To facilitate the application of identified RFS-related mRNAs in the clinical management of BC patients, the risk score for each patient was constructed with the regression coefficient from the univariate Cox analysis by using: Risk score = (3.002 × Expression EGFR + 4.514 × Expression TRPM4 + 5.360 × Expression TWIST1 + 3.590 × Expression ZEB1).