Results showed that the BIRC5, EPCAM, KRT18 and SPP1 genes were upregulated, and CDH11, TRPA1, TRPC1, TRPC3, TWIST1, VIM, and ZEB were downregulated whereas EGFR, TNC, TRPC6, TRPV6, TRPM4, UPK2, and VEGFA were not differently expressed in BC with respect to the normal tissue. This evidence concerns the gene TWIST1 and breast cancer.