When we examined the t-SNE plots clustered for all 68 proteins (Figure S1b) by the expression of the four proteins included in our multiprotein classifier, we found that the majority of the cancer samples expressed high levels of CA125 and HE4 (Figure S1c,d) and low levels of ITGAV and SEZ6L (Figure S1e,f). This evidence concerns the gene MUC16 and cancer.