PPARG and obesity due to melanocortin 4 receptor deficiency: In accordance with these studies, we found that HFD intake increased body weight, fat accumulation, and adipocyte size in the intra-abdominal compartment, as well as up-regulated the expression of C/EBP-α, PPAR-γ, and SREBP-1c genes in eWATs (Figure 1C–E, Figure 2A–C, and Figure 3A–E), indicating that HFD successfully induces obesity and its related mechanisms, including adipogenesis and lipogenesis, in WATs in mice.