In accordance with these studies, we found that HFD intake increased body weight, fat accumulation, and adipocyte size in the intra-abdominal compartment, as well as up-regulated the expression of C/EBP-α, PPAR-γ, and SREBP-1c genes in eWATs (Figure 1C–E, Figure 2A–C, and Figure 3A–E), indicating that HFD successfully induces obesity and its related mechanisms, including adipogenesis and lipogenesis, in WATs in mice. The gene discussed is SREBF1; the disease is obesity due to melanocortin 4 receptor deficiency.