In our study, the HDAC3-miR224-FOXA1 axis effectively regulated the migration and invasion of fibroblast cells under hypoxia, suggesting that the HDAC3-miR-224-FOXA1 axis may be involved in pulmonary fibrosis by regulating the migratory and invasive properties of human fibroblast cells under hypoxic conditions. This evidence concerns the gene FOXA1 and pulmonary fibrosis.