To test whether HDAC3 regulates the migration and invasion of human fibroblast cells under hypoxic conditions, we performed a scratch wound healing assay using diseased human lung fibroblasts from patients with idiopathic pulmonary fibrosis (DHLF-IPF cells) and MRC-5 human lung fibroblast cells. This evidence concerns the gene HDAC3 and pulmonary fibrosis.