PKD1 and urogenital neoplasm: To assess the potential of DST-PCR to detect homozygous indel mutations, we attempted to induce DSB in exon 11 of endogenous murine polycystic kidney disease 1 gene (Pkd1) after CRISPR/Cas9 gene editing using Pkd1 sgRNA in mIMCD-3 cells (Fig. 4a).