SOX2 and cancer: Results demonstrated that inhibition of PFKFB3 with PFK158 or genetic knockdown repressed the expression of phospho-PFKFB3 S461 and total PFKFB3 with decreased in expression of the cancer stem cell markers CD133, CD44, Aldh1, and Sox2 and also reduced expression of YAP/TAZ via upregulation of phospho/total LATS1 and MST1 (Fig. 7e).