To determine if PFKFB3 expression has selective impact on CSC enriched cells, we examined the cell viability effects of PFK158 on SCLC cells and observed a dose-dependent suppression of cell proliferation after 24 h for H1048, H1882, H1876, and DMS53 cells (IC50: H1048, 7.1 ± 1.6 μM; H1882, 8.4 ± 2.8 μM; H1876, 10.1 ± 2.3 μM; DMS53, 11.2 ± 2.4 μM). The gene discussed is PFKFB3; the disease is small cell lung carcinoma.