Anatomical appearance of flank-containing tumor shows that inhibition of PFKFB3 by PFK158 and genetic knockdown with shPFKFB3 regressed CSC tumor size noticeably with 0.353 ± 0.28 g and 0.135 ± 0.025 g of tumor respectively, when corresponded to that of untreated (1.80 ± 0.6 g) (Fig. 7c, d). The gene discussed is PFKFB3; the disease is neoplasm.