PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3) is a key regulator of high glycolytic flux in cancers by catalyzing the synthesis of F2,6P2 which allosterically activates PFK-1, the rate-limiting enzyme of glycolysis [24–27]. This evidence concerns the gene PFKFB3 and cancer.