We also evaluated the effect of APC-loss and KrasG12D on cholesterol metabolism-related gene expression in genetic CRC mice models (shRenilla, shApc and shApc-KrasG12D) (GSE67186), showing that PCSK9 and cholesterol biosynthesis genes were induced in shApc-KrasG12D compared to shRenilla or shApc mice colon (Fig. 1L). This evidence concerns the gene APC and colorectal carcinoma.