Since the results of our single-nucleus data analysis indicated abundant TNF-α/NFκB and TGF-β signaling in the MLC population and expression of these two cytokines in tumor-infiltrating microglia (Figs. 2 and 4 and Supplementary Fig. 3), we focused our analysis on the effect of these cytokines. This evidence concerns the gene TGFB1 and neoplasm.