This was in line with the results that the EGF receptor and the corresponding signalling pathways are highly enriched in kidney cancer cells (Figure S2A–C); (2) A83‐01, R‐spondin1, noggin and FGF10 were not required for all tested RCC organoids and, therefore, were excluded from the medium; (3) the omission of SB202190 caused RCC organoids to display hollow and cystic structures in ccRCC‐21_O and ccRCC‐29_O (Figure S1). The gene discussed is NOG; the disease is kidney cancer.