As previously mentioned, SIRT5 is involved in ammonia detoxificationthrough desuccinylation and the consequent inhibition of GLS, whichcatalyzes the hydrolysis of glutamine to glutamate and produces ammoniaas a byproduct.84 Notably, breast cancercells MDA-MB-231 and C2C12 overexpressing SIRT5 were characterizedby decreased ammonia levels, with a consequent reduction of ammonia-inducedautophagy and mitophagy (Figure 4). Here, GLS is linked to neoplasm.