Collectively, these resultssuggest that both the substrate sequence and the acyl modificationinfluence the compound inhibitory potency.160 Compound 11 was also tested in the CRC cell line HCT116,where it reduced SIRT5 levels, consequently decreased the activityof its substrate LDHB, and finally decreased autophagy and cell proliferation.142 Notably, 11 showed similar resultsin terms of its influence on SIRT5 expression, autophagy, and tumorgrowth in mouse xenograft models. This evidence concerns the gene LDHB and colorectal carcinoma.