The results showed that serum levels of TG, T-CHO, NEFAs, glycerol, LDL and HDL in high-fat diet WT rats were significantly higher than those of WT rats fed with normal diet, whereas these alterations were significantly reversed by AT1aR gene knockout, demonstrating that AT1aR knockout improved metabolic disorders in obese rats (Fig 1E–1J). This evidence concerns the gene AGTR1 and metabolic disease.