To further examine whether Shh signaling was required for BDDD-721 inhibition of medulloblastoma cell growth and induction of its apoptosis, DAOY and ONS-76 cells were treated with 10 μM cyclopamine (Shh signaling specific inhibitor), BDDD-721, and/or 0.6 μM SAG (Shh signaling specific agonist) for 48 h. Here, SAG is linked to medulloblastoma.