TLR1, TLR2, TLR3, TLR4, and TLR5 expression levels were high across the six immune subtypes in the pan-cancer data; TLR6, TLR7, TLR8, and TLR10 expression levels were moderate; and TLR9, TLR12P, and TLR8-AS1 expression levels were extremely low. The gene discussed is TLR4; the disease is cancer.