Consistent with the CRF-5-HT interactions mentioned above, CRF/CRF1 transmission regulates anxiety-related behaviors through 5-HT2R signaling in the PFC (Magalhaes et al., 2010), indicating that the CRF system interacts in the PFC with other neurotransmitters such serotonin and norepinephrine to regulate anxiety-like behaviors. This evidence concerns the gene CRHR1 and Anxiety.