All of the published studies on LDNs from active TB patients identified LDNs based on CD15 positivity and lower density buoyancy, but used varying multiplicities of infection, lengths of infection time, methods to stimulate the neutrophils before measuring NETosis, and ROS-sensitive fluorescent dyes (Deng et al., 2016; La Manna et al., 2019; Su et al., 2019), which may contribute to the differences in results. This evidence concerns the gene FUT4 and infection.