For instance, a study using pretreatment of Caco-2, a colon adenocarcinoma cell with EGF, showed a redistribution of TJPs, ZO-1, and occludin, while inhibition of MAPK/Erk has totally abolished the protective effects of EGF on tight junctions (TJs) [95]. This evidence concerns the gene EGF and colon adenocarcinoma.