For WAT and hypothalamus, our results showed a proportionate increase in FTO expression following the induction of obesity, and treatment with SMF, SAF, or SMF + SAF consistently downregulated FTO gene expression in a dose-dependent manner when compared to the untreated group, with the 6-week treatment duration being more effective than the 3-week duration (Figures 1 and 2). This evidence concerns the gene FTO and obesity due to melanocortin 4 receptor deficiency.