Compared to HCC patients without HBV infection, those with HBV infection were 8 years younger and showed higher proportions of AFP (≥20 ng/ml), the presence of liver cirrhosis, abnormal albumin (<40g/L), advanced BCLC stage, multiple tumor nodules, and vascular tumor thrombus as well as a higher male-to-female ratio. The gene discussed is AFP; the disease is cirrhosis of liver.