The multivariate Cox regression analysis indicated that HBV seropositivity (HR, 1.29; 95% CI, 1.10-1.21), incomplete tumor capsule (1.58; 1.41-1.77), vascular tumor thrombus (2.12; 1.90-2.36), tumor diameter (≥3 cm) (1.65; 1.29-2.12), more advanced BCLC stage (2.11; 1.85-2.41), AFP (≥20ng/ml) (1.69; 1.50-1.91), and direct bilirubin (>8μmol/L) (1.27; 1.11-1.45) independently contributed to shorter OS in HCC. This evidence concerns the gene AFP and hepatocellular carcinoma.