In the survival analysis for ICC, the multivariate Cox regression analysis indicated that more advanced BCLC stage (1.72; 1.08-2.72) and AFP (≥20ng/ml) (1.58; 1.00-2.49) were independently associated with shorter OS, while reoperation (0.12; 0.02-0.89) was independently associated with longer OS in ICC (Supplementary Table 3). The gene discussed is AFP; the disease is intrahepatic cholangiocarcinoma.