Successful expansion of tumor-reactive tumor-infiltrating lymphocytes (TIL) from lymph nodal metastasis of penile cancer patients, with 46.8% of CD8+ T cells and 45.4% from expanded TIL secreting IFN-γ in response to autologous tumor, supports the development of ACT strategies using TIL for the treatment of advanced and recurrent penile cancer (74). This evidence concerns the gene IFNG and penile cancer.