PPVs were 92.06%, 93.93%, 92.99%, 95.79%, and 91.12% when BALF melting curve method was performed in combination with LAM-TB, AFS, ADA, BALF culture, and BALF smear, respectively, which were significantly higher than the PPVs of BALF melting curve method alone and the combined use of two non-BALF melting curve method tests (p<0.05, respectively). This evidence concerns the gene ADA and tuberculosis.