Moreover, BALF melting curve method has a higher PPV with LAM-TB, AFS, ADA, BALF culture, or BALF smear as an add-on test (PPV =92.06%, 93.93%, 92.99%, 95.79%, and 91.12%, respectively), demonstrating a higher degree of sensitivity for PTB diagnosis compared with BALF melting curve method alone and the combined use of two non-BALF melting curve method tests. This evidence concerns the gene ADA and tuberculosis.