As shown in Figure 4, after treatment with Afatinib, the protein expressions of CEA, CA125, and CY-FRA21-1 in NSCLC cells were lower than that in DDP+PEM and saline groups, indicating that Afatinib can reduce the expressions of tumor markers, inhibit the growth of tumors, and improve the health status of patients with NSCLC. The gene discussed is MUC16; the disease is neoplasm.