Single-cell sequencing analysis also found that the gene expression profiles were different between the PD-1 high and PD-1 low subpopulations of CD8+ T cells in NSCLC, and that the transcription factor TOX can effectively predict the overall survival and PD-1 inhibitor efficacy in NSCLC [263]. The gene discussed is PDCD1; the disease is non-small cell lung carcinoma.