TIME can be divided into infiltrated–excluded (“Cold tumors”) in which CD8+ T cells were excluded from the tumor center, and infiltrated–inflamed (“Hot tumors”) in which an infiltrated–inflamed TIME was observed, as demonstrated by the increased expression of PD-L1 in tumors and highly activated CD8+ T cells expressing GRZB, IFN-γ, as well as the presence of infiltrated–tertiary lymphoid structures (TLSs) that are like lymph nodes, containing B cells, dendritic cells, and Treg cells [21]. This evidence concerns the gene CD274 and neoplasm.