In a recent study of scRNA-Seq analysis in 47 tumors from 36 NSCLC patients, the PD-1 inhibitor immunotherapy-responsive group had significantly increased numbers of precursor exhausted CD8+ T cells (Texp), featured by the low expression of co-suppressor molecules and high expression of GZMK [257]. This evidence concerns the gene PDCD1 and non-small cell lung carcinoma.