To systematically identify metabolic factors that induce the activation and differentiation of CD8+ T lymphocytes, we performed untargeted metabolomics on sorted splenic CD8+ T lymphocytes from the stroke models and demonstrated that the differential metabolites in perioperative stroke mice were mainly enriched in early citrate cycle (TCA cycle) metabolism, which is associated with glucose utilization and oxygen consumption [35]. Here, CD8A is linked to stroke disorder.