Compared with stroke-only mice (tMCAO group), the perioperative stroke mice (ICR + tMCAO group) exhibited significantly increased infarct volume by TTC staining (t(18) = 3.789, P = 0.0013, Fig. 1B) (3 days following ischemia) and by MAP2 staining (t(8) = 2.925, P = 0.0191, Fig. 1C) (7 days following ischemia). This evidence concerns the gene MAP2 and ischemia.