Cerebral mRNA expressions of Perforin (t(8) = 3.553, P = 0.0075) and Granzyme B (t(8) = 2.680, P = 0.0279) in perioperative stroke mice were obviously higher than stroke-only mice 7 days after stroke onset, whereas the mRNA level of IFN-γ (t(8) = 0.9686, P = 0.3611) and TNF-α (t(8) = 1.208, P = 0.2617) did not reflect abnormal variability (Fig. 3H). This evidence concerns the gene PRF1 and stroke disorder.