Furthermore, TNF-α-induced internalization was commonly observed in various human cancer cells, including non-small cell lung cancer (A549 and PC-9), colorectal cancer (DLD1) and glioblastoma (U87MG expressing EGFRvIII mutant), while spontaneous internalization was occurred efficiently in PC-9 cells (Fig. 2e). Here, TNF is linked to colorectal cancer.