In summary, the methylated DNA regions highlighted with our approach, are potential useful biomarkers for HCC and may also reveal important biological information, specifically ZNF518B and its associated DMR, Chr10:133,445,694–133,446,718, is among the most important features and has been previously described with possible implications in cancer cell invasion and metastatic potential81. This evidence concerns the gene ZNF518B and hepatocellular carcinoma.