In addition, STn has been found on plasminogen in serum frompatients with intestinal metaplasia and gastritis.366 Plasminogen O-glycans were analyzed bynano-HPLC-MALDI-TOF/TOF after lysine-sepharose affinity chromatography,in-gel de-O-glycosylation by reductive β-eliminationand permethylation.366 A recent study thatexplored the serum protein O-glycosylation in 62CRC patients and 20 healthy individuals by Gizaw et al.367 has revealed differences in O-glycan patterns between cancer samples and healthy controls thatcorrelated with cancer progression. This evidence concerns the gene EEF1A2 and cancer.