To further explore the content of tumor-infiltrating TH cell subtypes in breast cancer tissues, we performed immunofluorescence staining on paraffin tissue sections of primary foci from 30 TNBC patients, in which CD4+T-bet+ TH1, CD4+GATA3+ TH2, and CD4+CD25+ Treg cells were labeled (see Supplementary Table S1 for clinical information and corresponding TH cell content of these patients). This evidence concerns the gene GATA3 and breast carcinoma.