IFNG and central nervous system cancer: T cell medium (TCM), chicken ovalbumin (OVA), VV peptide B8R, mouse pancreatic cancer cell line DT6606, mouse glioma cell line GL261, and lysed mouse glioma GL261 cells were used for ex vivo stimulation of splenocytes harvested on days 7, 14, and 21 post-treatment from treated mice and IFN-γ production determined 72 h later.