Second-generation (CD28/CD3ζ) and third-generation (CD28/4-1BB/CD3ζ) TEM8-CARs have been engineered to co-target TNBC cells expressing TEM8 as well as tumor-associated vessels, demonstrating the ability to induce TNBC cell regression, as well as to reduce tumor neoangiogenesis in the xenograft mouse model (112, 113). This evidence concerns the gene TNFRSF9 and neoplasm.