To further support the role of STING in TOP1 poison-induced immune gene expression, we determined immune gene activation by LMP776 and CPT in a different cell model, the murine melanoma B16 cell line, by using STING gene wild-type (wt) or CRISPR-KO B16 cell lines (Fig. 4b) [13]. The gene discussed is TOP1; the disease is melanoma.