In old APP/PS1 mice, amyloid was observed in EPS regions, as shown in an orthogonal confocal image (Supplementary Fig. 7), and was seen in all PAS types (Fig. 7e, g; Supplementary Figs. 8–11) including in peri-arterial and peri-arteriolar areas that lacked any significant pial coverage (Fig. 7f–h; Supplementary Figs. 10 and 12). Here, PSEN1 is linked to amyloidosis.