In an experimental rat model of stroke, hyperbaric oxygen treatment helped protect the BBB, potentially by regulating the ATP/NAD+/Sirt1 signaling pathway.164 Similarly, quercetin has been shown to protect the BBB and alleviate ischemia–reperfusion-induced injuries via activation of Sirt1 signals in rats.395 Minocycline has also been shown to ameliorate hypoxia-induced BBB disruption. This evidence concerns the gene SIRT1 and Stroke.