Both host and microbe transcriptomic information was collected within microscopically defined regions of interest based on immunofluorescent imaging of specific cell types stained for cell surface markers: tumor cells (panCK+), T cells (CD3+CD45+), macrophages (CD68+CD45+), other immune cells (CD3−CD68−CD45+), and tumor stroma (autofluorescence+, panCK−CD45−) (figure 1B). This evidence concerns the gene CD68 and neoplasm.