We then evaluated production of CCL4 by various tumor cells, including murine tumor cell lines (MC38, CT26, and 4T1), a human cancer cell lines (HT29 colon cancer and 1861mel melanoma) in vitro, and attempted to clarify the mechanisms for the inhibition of CCL4 production in tumor cells by SCD1 inhibition. This evidence concerns the gene CCL4 and melanoma.