While T-cell, CD8 T-cell, cytotoxic lymphocyte, and B-cell lineage scores all closely aligned to the purified leukocyte population as expected, we did observe signaling indicative of NK cells, myeloid dendritic cells, and neutrophils in non-leukocyte populations, suggesting that there was some crossover in these specific populations during cell sorting for epithelial cells (EPCAM+), leukocytes (CD45+), fibroblasts (FAP+), and endothelial cells (CD31+), or that the signatures cannot be used for precise enumeration of these lineages in CRC tissue. The gene discussed is PECAM1; the disease is colorectal carcinoma.