qRT-PCR was performed to verify the expression levels of CXCR2, PTPRC, CCR2, MNDA, NCF2, S100A9, S100A8 and S100A12. The expression levels of CXCR2, CCR2, NCF2, S100A9, S100A12 in NAFLD cell model were significantly higher than control group. The gene discussed is S100A8; the disease is metabolic dysfunction-associated steatotic liver disease.