qRT-PCR was performed to verify the expression levels of CXCR2, PTPRC, CCR2, MNDA, NCF2, S100A9, S100A8 and S100A12. The expression levels of CXCR2, CCR2, NCF2, S100A9, S100A12 in NAFLD cell model were significantly higher than control group. This evidence concerns the gene S100A9 and metabolic dysfunction-associated steatotic liver disease.