Tbx1 and Car3 have also been shown for the OM and are expressed in our dataset22,23, together with additional gene markers for the OM population, amongst which Prrx1 and Twist1 (Fig. 1b, 1145 genes found differentially expressed in OM cluster compared to the E16.5 SGN clusters, Supplementary Fig. 1). The gene discussed is TWIST1; the disease is ocular melanoma.