Transient silencing of MTHFD2 influences primary cellular events such as proliferation, apoptosis and migration by inhibiting several downstream molecules, including MCM4, MCM7, Cyclin D1, and ATP5G3, as well as Zeb1, Vimentin and Snail, which contribute the cancer cell growth and epithelial-to-mesenchymal transition (EMT). This evidence concerns the gene ZEB1 and cancer.