APC and metastatic colorectal cancer: In colorectal cancer, the combined mutation frequency of APC-PTPRT, APC-PTPRT-TP53, and PTPRT-TP53 was significantly higher in metastatic colorectal cancer than in primary cancer (Figure 4(b); q = 3.4E − 05, q = 0.0006, and q = 2.6E − 06, respectively).