Here, we further validated scFEA on the curated iron ion metabolic modules by applying the method on our recently collected scRNA-seq data of 168 patient-derived pancreatic cancer cell lines Pa03c under four conditions: normoxia (N), hypoxia (H), normoxia and knockdown of APEX1 (N-APEX1-KD), and hypoxia and knockdown of APEX1 (H-APEX1-KD). Here, APEX1 is linked to familial pancreatic carcinoma.